Journal: Journal of Nanomaterials

Article Title: Biofunctionalized Magnetic Nanoparticles with Multiplex Touchdown PCR for Simultaneous and Rapid Detection/Identification of Campylobacter jejuni and Campylobacter coli

doi: 10.1155/2022/5104187

Figure Lengend Snippet: Figure 1: Schematic illustrating the functionalization of anti-MEA FMNs (a) and the overall procedure of IMS–multiplex TD–PCR assay to detect C. jejuni and C. coli cells (b).

Article Snippet: Four reference strains of C. jejuni (ATCC 33560 and DMST 29021) and C. coli (NTCC 11353 and DMST 28030) were included in this study.

Techniques: Multiplex Assay

Journal: Journal of Nanomaterials

Article Title: Biofunctionalized Magnetic Nanoparticles with Multiplex Touchdown PCR for Simultaneous and Rapid Detection/Identification of Campylobacter jejuni and Campylobacter coli

doi: 10.1155/2022/5104187

Figure Lengend Snippet: Figure 5: SEM images of anti-MEA FMNs at 40000× (a) and 90000× (b) magnification showing cube-like FMNs. The scale bars represent 1 μm and 500 nm, respectively. Aggregation of bacterial cells surrounded with anti-MEA FMNs for C. jejuni (c) and C. coli (d) cells. Some small part of the cell surface was free from the anti-MEA FMNs, as indicated by an arrow. The bar represents 2 μm.

Article Snippet: Four reference strains of C. jejuni (ATCC 33560 and DMST 29021) and C. coli (NTCC 11353 and DMST 28030) were included in this study.

Techniques:

Journal: Journal of Nanomaterials

Article Title: Biofunctionalized Magnetic Nanoparticles with Multiplex Touchdown PCR for Simultaneous and Rapid Detection/Identification of Campylobacter jejuni and Campylobacter coli

doi: 10.1155/2022/5104187

Figure Lengend Snippet: Figure 6: IMS–multiplex TD–PCR assays of single pure cultures of C. jejuni (a), C. coli (b), and mixed cultures (c). Negative control: no template lane; positive control: mixed C. jejuni and C. coli DNA templates.

Article Snippet: Four reference strains of C. jejuni (ATCC 33560 and DMST 29021) and C. coli (NTCC 11353 and DMST 28030) were included in this study.

Techniques: Multiplex Assay, Negative Control, Positive Control

Journal: Journal of Nanomaterials

Article Title: Biofunctionalized Magnetic Nanoparticles with Multiplex Touchdown PCR for Simultaneous and Rapid Detection/Identification of Campylobacter jejuni and Campylobacter coli

doi: 10.1155/2022/5104187

Figure Lengend Snippet: Figure 7: Inclusivity tested by IMS–multiplex TD–PCR assay. Lane M, DNA ladder; lanes 1-23, C. jejuni FSC strain No. 1-23; lanes 24-25, C. jejuni ATCC 33560 and DMST 29021, respectively; lanes 26-48, C. coli FSC strain No. 1-23; lanes 49-50, C. coli NTCC 11353 and DMST 28030, respectively.

Article Snippet: Four reference strains of C. jejuni (ATCC 33560 and DMST 29021) and C. coli (NTCC 11353 and DMST 28030) were included in this study.

Techniques: Multiplex Assay

Journal: Journal of Nanomaterials

Article Title: Biofunctionalized Magnetic Nanoparticles with Multiplex Touchdown PCR for Simultaneous and Rapid Detection/Identification of Campylobacter jejuni and Campylobacter coli

doi: 10.1155/2022/5104187

Figure Lengend Snippet: Figure 9: Detection C. jejuni and C. coli by IMS–multiplex TD–PCR assays of spiked chicken breast samples at different concentrations of mixed contamination (ratio 1 : 1) from 107 to 100 CFU/g. Lane, (-) negative control, and (+) positive control.

Article Snippet: Four reference strains of C. jejuni (ATCC 33560 and DMST 29021) and C. coli (NTCC 11353 and DMST 28030) were included in this study.

Techniques: Multiplex Assay, Negative Control, Positive Control

Journal: Frontiers in Microbiology

Article Title: Molecular Characterization and Antibiotic Resistant Profiles of Campylobacter Species Isolated From Poultry and Diarrheal Patients in Southeastern China 2017–2019

doi: 10.3389/fmicb.2020.01244

Figure Lengend Snippet: Dendrogram of 80 C. jejuni strains based on Sma I-mediated PFGE profiles. PFGE pattern, strain source, isolation year, sequence type, and clonal complex are depicted. Asterisk indicates newly designated ST in this study. Black squares of the antibiogram indicate resistance to antibiotics.

Article Snippet: C. jejuni ATCC 33560 was used as a control organism.

Techniques: Isolation, Sequencing

Journal: Applied and Environmental Microbiology

Article Title: Active Packaging of Immobilized Zinc Oxide Nanoparticles Controls Campylobacter jejuni in Raw Chicken Meat

doi: 10.1128/aem.01195-20

Figure Lengend Snippet: Figure 3. The count of Campylobacter jejuni cocktail (F38011, Human10, 1173, and ATCC 33560) 814

Article Snippet: Each piece of raw chicken breast was then placed 492 into a sterile Petri dish and inoculated with a cocktail of four C. jejuni strains (i.e., F38011, 493 Human 10, ATCC 33560, 1173).

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Active Packaging of Immobilized Zinc Oxide Nanoparticles Controls Campylobacter jejuni in Raw Chicken Meat

doi: 10.1128/aem.01195-20

Figure Lengend Snippet: Figure 7. Interaction network of genes associated with outer membrane synthesis in C. jejuni 848

Article Snippet: Each piece of raw chicken breast was then placed 492 into a sterile Petri dish and inoculated with a cocktail of four C. jejuni strains (i.e., F38011, 493 Human 10, ATCC 33560, 1173).

Techniques: Membrane

Journal: BMC Microbiology

Article Title: Specific detection of Brucella spp. from slaughter-aged livestock using a dual priming oligonucleotide system

doi: 10.1186/s12866-025-03807-w

Figure Lengend Snippet: The bacterial strains used in the study

Article Snippet: Campylobacter jejuni , ATCC 33,560 , Lactobacillus casei , ATCC 393.

Techniques:

Journal: BMC Microbiology

Article Title: Specific detection of Brucella spp. from slaughter-aged livestock using a dual priming oligonucleotide system

doi: 10.1186/s12866-025-03807-w

Figure Lengend Snippet: Specificity of the DPO-based PCR assay for Brucella spp. The DPO-based PCR assay developed in this work has high diagnostic specificity for Brucella spp. ( a ), while conventional primer-based PCR assay shows a low specificity ( b ). M: DNA Marker 2000; Lanes 1–24 were B. abortus , B. melitensis , A hydrophila , B cereus , C jejuni , C. sakazakii , C. perfringens , E. coli , K. pneumoniae , L. monocytogenes , L. welshimeri , L. casei , L. plantarum , P. aeruginosa , P. vulgaris , S. aureus , S. enteritidis , S. flexneri , S. haemolyticus , V. parahaemolyticus , V. cholerae , V. anguillarum , V. vulnificus and Y. enterocolitica , respectively

Article Snippet: Campylobacter jejuni , ATCC 33,560 , Lactobacillus casei , ATCC 393.

Techniques: Diagnostic Assay, Marker

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: Effect of garlic concentrates on the growth and survival of Campylobacter jejuni in sterilized saline water at different temperatures and times a

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques: Saline

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: Effect of garlic concentrate on the growth and survival of Campylobacter jejuni in sterilized campylobacter nonselective broth at different temperatures and times

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: (a) Raw spectra of a C. jejuni IR transmission spectrum (line 1, green) and micro-Raman spectrum (line 2, red) with an excitation wavelength of 532 nm. (b) Micrograph of bacterial samples. The black frame indicates the mapping area for taking the Raman scans shown in panel a. (c) Raman mapping experiment of a single C. jejuni cell at selective wave numbers, namely, 713 to 780 cm−1 (image a), 1,084 to 1,200 cm−1 (image b), 1,272 to 1,388 cm−1 (image c), 1,518 to 1,661 cm−1 (image d), and 2,788 to 3,033 cm−1 (image e). cts, photon counts.

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques: Transmission Assay

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: (a) Second-derivative transformation of FT-IR spectral features of C. jejuni treated with 5 μM diallyl sulfide at 4°C for different time intervals (black, 0 h; indigo, 1 h; blue, 3 h; green, 5 h; red, 24 h). (b) Second-derivative transformation of FT-IR spectral features of C. jejuni treated with 25 μl/ml garlic concentrate at 4°C for different time intervals (green, 0 h; blue, 1 h; indigo, 3 h; black, 5 h; red, 24 h). The red columns show differences in spectral features treated with garlic concentrate and organosulfur compounds.

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques: Transformation Assay

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: Second-derivative transformation of Raman spectral features of C. jejuni treated with 5 μM diallyl sulfide at 4°C for different time intervals (blue, treated for 4 h; black, control). The dotted red columns show differences of spectral features between FT-IR and Raman spectroscopies (especially sulfur compounds).

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques: Transformation Assay, Control

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: Transmission electron microscope images of Campylobacter jejuni without (a) and with (b) treatment with organosulfur compounds (diallyl sulfide) derived from garlic (Allium sativum).

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques: Transmission Assay, Microscopy, Derivative Assay

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: (a) Representative two-dimensional principal-component analysis of C. jejuni cocktail in sterilized campylobacter nonselective broth with 5 μM diallyl sulfide treatment for 0 (control), 2, 4, 8, 12, and 24 h at room temperature (22°C) using infrared spectra. (b) Representative dendrogram analysis of C. jejuni cocktail in sterilized campylobacter nonselective broth with 5 μM diallyl sulfide treatment for 0 (∧), 4 (&), 12 (*), and 24 h (#) at room temperature (22°C) using Raman spectra.

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques: Control

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: Partial least-squares regression models for quantification of live C. jejuni strains treated with garlic-derived organosulfur compounds a

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Investigating Antibacterial Effects of Garlic ( Allium sativum ) Concentrate and Garlic-Derived Organosulfur Compounds on Campylobacter jejuni by Using Fourier Transform Infrared Spectroscopy, Raman Spectroscopy, and Electron Microscopy ^▿ †

doi: 10.1128/AEM.02845-10

Figure Lengend Snippet: Loading plots of first principal component obtained from PLSR for FT-IR spectroscopy (a) and Raman spectroscopy (b) for C. jejuni stress.

Article Snippet: Four C. jejuni strains (ATCC 33291, ATCC 33560, ATCC 43430, and ATCC 49943) were obtained from Microbiologics, Inc. (St.

Techniques: IR Spectroscopy, Raman Spectroscopy